Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching

Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching

Blog Article

Abstract Real-time visualisation of chick embryo development occurring inside an opaque eggshell is a major goal of developmental biology.This goal was partially achieved when 3-day-old embryos recovered from fertilised shelled eggs, preincubated in a conventional incubator, hatched after the egg contents had been transferred into shell-less embryo culture systems constructed of transparent plastic film placed in a plastic cup.However, the hatchable shell-less embryo culture systems CALM CHAI LATTE REISHI described thus far cannot be used to visualise the dynamic morphological changes associated with the emergence of major organ systems during the first 3 days of embryo development, as the blastoderm area needs to be covered with an opaque membrane.

Here, we present the development of a chick Wound Treatments embryo culture system that enables omnidirectional real-time visualisation of the developing embryo, beginning from the newly laid blastoderm stage embryo until hatching.